Fig. 5. RGD-to-RGE change in fibulin-5 increases the capacity of 4T1 cells to form subcutaneous tumors. Top, graphical representation of the tumor volumes formed by the indicated 4T1 cells when injected in the flank of BALB/c mice. Tumor volumes were measured for six weeks. Bottom, western-blot analysis of the fibulin-5 expression in three tumors formed by 4T1 Fibulin-5KD cells, four 4T1 Fibulin-5RGE cells and one control cells. Molecular weight marker is indicated on the right and β-actin was employed as loading control.